PathHunter® ADRB2 Total GPCR Internalization HEK 293 Cell Line

Background Information:

The PathHunter® ADRB2 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. isoproterenol) based internalization of ADRB2 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged ADRB2 and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADRB2 receptor to the early endosome leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Coupling:

Gs

Protein Name:

beta2

Protein Aliases:

Beta-2 adrenoceptor

Accession Number:

NM_000024

UniProt Number:

P07550

Gene Name:

ADRB2

Gene ID:

154

Gene Aliases:

ADRBR|BAR|B2AR

Target Species:

Human

Cell Background:

HEK293