Biological Information
Background Information:
The KILR® Retroparticles for Adherent & Suspension Cells (Hygromycin B) are MMLV particles designed to transduce adherent and suspension cells respectively. This allows production of PL-tagged, hygromycin B-resistant target cells of interest for use in cytotoxicity assays involving detection of cell lysis. This product uses EFC technology (https://www.discoverx.com/technologies-platforms/enzyme-fragment-complementation-technology). The retroparticle mix can be used to transduce target cells with a PL-tagged housekeeping gene. Target cell death in a cytotoxicity assay will release PL to the media, where EA contained in the detection reagent will produce EFC signal. These retroparticles ship as 2 vials each of cryopreserved adherent and suspension retroparticles.
Usage
Product Type:
Retroparticles
Application:
Cytotoxicity
Storage Conditions:
Short term (24 h): Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Measures:
Cytotoxicity
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
KILR®