Biological Information
Background Information:
The ChemiSCREEN™ Rhesus Macaque CCR5 Chemokine Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. MIP, RANTES) based activation of CCR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR5 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CCR5 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus macaque version of the protein.
Target Class:
GPCR
Family:
Chemokine
Sub Family:
Class A
Protein Name:
CCR5
Protein Aliases:
CC-CKR-5|CCR-5|CCR5|CHEMR13|HIV-1 fusion coreceptor|CD antigen CD195
Accession Number:
NM_001042773.2
UniProt Number:
P51682
Gene Name:
CCR5
Gene ID:
735311
Gene Aliases:
CKR-5|CC-CKR-5|CKR5|CD195|IDDM22
Target Species:
Rhesus Macaque
Cell Background:
Chem-1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiSCREEN™