Ready-to-Assay™ CCR4 Chemokine Receptor Frozen Cells

The Ready-to-Assay™ CCR4 Chemokine Receptor Frozen Cells measure CCR4 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.

Catalog #: HTS009RTA Size: 2 vials


Biological Information
Background Information:
The Ready-to-Assay™ CCR4 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. MIP, RANTES) based activation of CCR4 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCR4 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR4 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:
Chemokine Receptors
Accession Number:
Target Name:
Target Aliases:
Chemokine (C-C motif) receptor 4, CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5
Target Species:
Cell Background:
Product Type:
Frozen Cells
Drug Discovery & Development
Storage Conditions:
Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Measures:
Calcium Flux
Detection Method:
Additional Information

User Manuals & Protocols

70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1

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HTS009RTA Datasheet

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