Biological Information
Background Information:
The PathHunter® U2OS SMO(W535L) MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting SMO. This cell line enables assessment of chaperone-based stabilization of SMO(W535L) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant SMO(W535L), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant SMO, it is trafficked to the membrane, producing EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Sub Family:
Frizzled
Coupling:
Gi/Go
Protein Name:
SMO
Protein Aliases:
Protein Gx
Accession Number:
NM_005631.4
UniProt Number:
Q99835
Gene Name:
SMO
Gene ID:
6608
Gene Aliases:
SMOH
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Pharmacotrafficking
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Pharmacotrafficking
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®