Biological Information

Background Information:

The PathHunter® U2OS SMO(W535L) MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting SMO. This cell line enables assessment of chaperone-based stabilization of SMO(W535L) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant SMO(W535L), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant SMO, it is trafficked to the membrane, producing EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Sub Family:

Frizzled

Coupling:

Gi/Go

Protein Name:

SMO

Protein Aliases:

Protein Gx

Accession Number:

NM_005631.4

UniProt Number:

Q99835

Gene Name:

SMO

Gene ID:

6608

Gene Aliases:

SMOH

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Pharmacotrafficking

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Pharmacotrafficking

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®