PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay

The PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant KCNH2 GPCR via detection of localization to the membrane. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1064C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting KCNH2. This cell line enables assessment of chaperone-based stabilization of KCNH2(G601S) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant KCNH2(G601S), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant KCNH2, it is trafficked to the membrane, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Ion Channel
Family:
Potassium Ion Channel
Sub Family:
Voltage-Gated
Accession Number:
NM_000238
Target Name:
KCNH2(G601S)
Target Aliases:
Potassium voltage-gated channel, subfamily H (eag-related), member 2
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Pharmacotrafficking
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Pharmacotrafficking
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-302 PathHunter Pharmacotrafficking Assays REV4 User Manual

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Datasheets

93-1064C3 Datasheet

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