PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay

The PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay measures pharmacochaperone-based stabilization of mutant KCNH2 GPCR via detection of localization to the membrane. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-1064C3 Size: 2 vials

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Biological Information
Background Information:	The PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting KCNH2. This cell line enables assessment of chaperone-based stabilization of KCNH2(G601S) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant KCNH2(G601S), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant KCNH2, it is trafficked to the membrane, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	Ion Channel
Family:	Potassium Ion Channel
Sub Family:	Voltage-Gated
Accession Number:	NM_000238
Target Name:	KCNH2(G601S)
Target Aliases:	Potassium voltage-gated channel, subfamily H (eag-related), member 2
Target Species:	Human
Cell Background:	U2OS

Usage
Product Type:	Stable Cell Lines
Application:	Pharmacotrafficking
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	Pharmacotrafficking
Detection Method:	Chemiluminescence

Additional Information
Brand:	PathHunter®