PathHunter® U2OS BLK Activity Assay

The PathHunter® U2OS BLK Activity Assay measures BLK CTK activity via ligand-induced SH2 domain protein recruitment. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0882C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® U2OS BLK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting CTKs. This cell line enables assessment of CTK activation via detection of resulting GHR phosphorylation-induced SH2 domain protein recruitment, mediated by BLK-JAK2 fusion protein. This product uses EFC technology. The included cell line overexpresses PK-tagged relevant cytokine receptor and EA-tagged SH2 domain protein alongside an engineered fusion of the cytokine receptor binding domain of JAK2 (Janus Kinase 2) to the catalytic domain from the CTK of interest, BLK. Upon activation of the cytokine receptor, the CRBD-CTK fusion phosphorylates the receptor, leading to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
Kinase
Family:
Cytosolic Tyrosine Kinase
Sub Family:
Protein Tyrosine
Accession Number:
NM_000163
Target Name:
Blk
Target Aliases:
Growth hormone receptor, MODY11
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Functional Activity
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-249 PathHunter Cytosolic Tyrosine Kinase Activity Assay REV4 User Manual

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Datasheets

93-0882C3 Datasheet

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