PathHunter® Sargramostim Bioassay Kit

The PathHunter® Sargramostim Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses GM-CSF or Sargramostim-induced dimerization of CSF2RB and CSF2RA receptors.

View bioassay qualification data.

Catalog #: 93-1078Y3-00111

$1,989.00$9,979.00


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Biological Information
Background Information:
The PathHunter® Sargramostim Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses GM-CSF or Sargramostim-induced dimerization of CSF2RB and CSF2RA receptors. This product uses EFC technology. The cell line included overexpresses CSF2RB target protein fused to PK alongside CSF2RA target protein fused to EA. Upon ligand-induced dimerization of CSF2RB and CSF2RA, detectable EFC signal is produced. This assay has been optimized and qualified with Leukine® (not supplied in the bioassay kit). Leukine® is a registered trademark of Genzyme Corporation (licensed). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.
Target Class:
Cytokine Receptor
Family:
Receptor Tyrosine Kinase
Accession Number:
NM_000395.2
Target Name:
CSF2Rb/CSF2RA
Target Aliases:
CD131, CDw131, IL3RB, IL5RB, SMDP5, betaGMR, CD116, CDw116, CSF2R, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF-R-alpha, GMCSFR, GMCSFR-alpha, GMR, GMR-alpha, SMDP4, alphaGMR, CSF2RB protein
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Bioassay Kits
Application:
Potency; Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
Dimerization
Detection Method:
Chemiluminescence
Bioassay Data
Qualified With:
Leukine®
Trademark Statement:
Leukine® is a registered trademark of Genzyme Corporation (licensed)
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-398_PathHunter_Sargramostim_User Manual_Rev1

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Datasheets

93-1078Y3x Datasheet

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