PathHunter® CHRM5 Activated GPCR Internalization U2OS Cell Line

The PathHunter® CHRM5 Activated GPCR Internalization U2OS Cell Line measures CHRM5 (GPCR) internalization via early-endosome localization. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0657C3 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® CHRM5 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. acetylcholine) based internalization of CHRM5 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged CHRM5. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting CHRM5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Acetylcholine Receptor (Muscarinic)
Sub Family:
Class A
Coupling:
Gq
Accession Number:
AF498919
Target Name:
CHRM5
Target Aliases:
Cholinergic receptor, muscarinic 5, HM5, CHRM5, M5, Acetylcholine (Muscarinic)
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
Internalization
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-259 PathHunter Activated GPCR Internalization Assay REV6 User Manual

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Datasheets

93-0657C3 Datasheet

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