PathHunter® CHO-K1 PTAFR β-Arrestin Cell Line

The PathHunter® CHO-K1 PTAFR β-Arrestin Cell Line measures PTAFR (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0236C2 Size: 2 vials
Inquire
Biological Information
Background Information:
The PathHunter® PTAFR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged PTAFR and EA-tagged β-Arrestin 2. Activation of PTAFR stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Platelet-Activating Factor Receptor
Sub Family:
Class A
Coupling:
Gi/Go & Gq
Accession Number:
NM_000952.4
Target Name:
PTAFR
Target Aliases:
Platelet activating receptor, PAFR
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual

View Document

Datasheets

93-0236C2 Datasheet

View Document
AssayComplete™ Cell Detachment Reagent
92-0009
50 mL

$109.00

AssayComplete™ Cell Plating 2 Reagent
93-0563R2A
100 mL

$339.00

PathHunter® Detection Kit
93-0001
Inquire
AssayComplete™ Freezing Reagent F2
92-5102FR
2 x 50 mL

$339.00

AssayComplete™ Thawing Reagent T2
92-4102TR
100 mL

$249.00

AssayComplete™ Cell Culture Kit-107
92-3107G
Kit

$397.00