PathHunter® CHO-K1 cyno MRGPRX2 β-Arrestin Cell Line

The PathHunter® CHO-K1 cyno MRGPRX2 β-Arrestin Cell Line measures cMRGPRX2 (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells. Cyno protein.

Catalog #: 93-1134C2 Size: 2 vials
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Biological Information
Background Information:
The PathHunter® cMRGPRX2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAMP-20) based activation of cMRGPRX2 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged cMRGPRX2 and EA-tagged β-Arrestin 2. Activation of cMRGPRX2 stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Cyno version of the protein.
Target Class:
GPCR
Family:
Class A Orphans
Coupling:
Gq
Accession Number:
XM_015435307.1
Target Name:
MRGPRX2
Target Aliases:
MAS-related GPR, member X2, cynomulgas
Target Species:
Cynomolgus Monkey
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual

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Datasheets

93-1134C2 Datasheet

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