PathHunter® CHO-K1 CALCR-RAMP2 (AMY2) β-Arrestin Cell Line

The PathHunter® CHO-K1 CALCR-RAMP2 (AMY2) β-Arrestin Cell Line measures CALCR (GPCR) activity via recruitment of β-Arrestin2. This cell line ships as two vials of cryopreserved cells.

Catalog #: 93-0267C2 Size: 2 vials
Inquire
Biological Information
Background Information:
The PathHunter® CALCR-RAMP2(AMY2) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. calcitonin, amylin) based activation of CALCR GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged CALCR and EA-tagged β-Arrestin 2. Activation of CALCR stimulates the recruitment of β-Arrestin 2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Both calcitonin and amylin activate amylin receptors (AMY1, AMY2, AMY3). Amylin, a 37-amino acid peptide hormone, is primarily synthesized in pancreatic beta cells and co-secreted with insulin in response to ingested nutrients. Amylin receptors are GPCRs composed of two units: a core unit formed by the calcitonin receptor (a 7-transmembrane receptor) and one of three receptor activity-modifying proteins (RAMP1-3). Dual amylin and calcitonin receptor agonists have shown significant efficacy in promoting body weight loss, enhancing glucose tolerance, and reducing fat accumulation in liver tissue. Moreover, Amylin and GLP-1R agonists are believed to function via comparable yet distinct mechanisms, particularly in regulating appetite. Consequently, Amylin-based therapeutics exhibit promising potential in combating obesity.
Target Class:
GPCR
Family:
Calcitonin Receptor
Sub Family:
Class B
Coupling:
Gs
Accession Number:
NM_001742.2
Target Name:
CALCR-RAMP2(AMY2)
Target Aliases:
Calcitonin receptor, CRT, CT-R, CTR, CTR1, Amylin receptor, AMY2
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
β-Arrestin isoform:
β-Arrestin-2
Assay Measures:
β-Arrestin Recruitment
Detection Method:
Chemiluminescence
Additional Information
Brand:
PathHunter®

User Manuals & Protocols

70-247 PathHunter Beta-Arrestin Assay for GPCR Cell Lines REV5 User Manual

View Document

Datasheets

93-0267C2 Datasheet

View Document
AssayComplete™ Thawing Reagent T2
92-4102TR
100 mL

$249.00

AssayComplete™ Cell Culture Kit-107
92-3107G
Kit

$397.00

AssayComplete™ Cell Plating 2 Reagent
93-0563R2A
100 mL

$339.00

AssayComplete™ Cell Detachment Reagent
92-0009
50 mL

$109.00

AssayComplete™ Freezing Reagent F2
92-5102FR
2 x 50 mL

$339.00

PathHunter® Detection Kit
93-0001
Inquire