cAMP Hunter™ CHO-K1 HTR1B Gi cell Line

Background Information:

The cAMP Hunter™ HTR1B Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR1B GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HTR1B , a naturally Gi/Go coupled GPCR. Upon activation of HTR1B , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

5-HT1B

Protein Aliases:

5-HT1B|S12|Serotonin 1D beta receptor|5-HT-1D-beta|Serotonin receptor 1B

Accession Number:

NM_000863

UniProt Number:

P28222

Gene Name:

HTR1B

Gene ID:

3351

Gene Aliases:

S12|5-HT1B|HTR1D2|5-HT1DB

Target Species:

Human

Cell Background:

CHO-K1