Biological Information
Background Information:
The PathHunter® IL2RB/IL2RG/IL2RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-2) induced dimerization of IL2RB/IL2RG/IL2RA receptors. This product uses EFC technology. The cells included overexpress IL2RB target protein fused to PK alongside IL2RG target protein fused to EA and untagged ILR2A. Upon ligand-induced dimerization of IL2RB/IL2RG/IL2RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Cytokine Receptor
Family:
IL-2 Receptor
Protein Name:
IL2RB
Protein Aliases:
IL-2R subunit beta|IL-2RB|High affinity IL-2 receptor subunit beta|Interleukin-15 receptor subunit beta|p70-75|p75|CD antigen CD122
Accession Number:
NM_000878.3
UniProt Number:
P14784
Gene Name:
IL2RB
Gene ID:
3560
Gene Aliases:
IL15RB
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
Assay Measures:
Dimerization
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
PathHunter®