PathHunter® NTSR1 Activated GPCR Internalization U2OS Cell Line

Background Information:

The PathHunter® NTSR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. neurotensin) based internalization of NTSR1 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged NTSR1. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting NTSR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Neurotensin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

NT1

Protein Aliases:

NTR1|High-affinity levocabastine-insensitive neurotensin receptor|NTRH

Accession Number:

AY429106

UniProt Number:

P30989

Gene Name:

NTSR1

Gene ID:

4923

Gene Aliases:

NTR|NTRR

Target Species:

Human

Cell Background:

U2OS