Biological Information

Background Information:

The PathHunter® LTB4R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged LTB4R and EA-tagged β-Arrestin 2. Activation of LTB4R stimulates the recruitment of β-Arrestin 2 and produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Leukotriene

Sub Family:

Class A

Coupling:

Gi/Go & Gq

Protein Name:

BLT1 (LTB4)

Protein Aliases:

LTB4-R1|Chemoattractant receptor-like 1|G-protein coupled receptor 16|P2Y purinoceptor 7|P2Y7

Accession Number:

AY322535

UniProt Number:

Q15722

Gene Name:

LTB4R

Gene ID:

1241

Gene Aliases:

BLT|BLT1|BLTR|CMKRL1|GPR16|P2RY7

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®