ChemiSCREEN™ M5 Acetylcholine Muscarinic Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ M5 Acetylcholine Muscarinic Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of CHRM5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM5 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM5 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Acetylcholine (Muscarinic)

Sub Family:

Class A

Protein Name:

M5

Protein Aliases:

acetylcholine receptor, muscarinic 5

Accession Number:

NM_012125

UniProt Number:

P08912

Gene Name:

CHRM5

Gene ID:

1133

Target Species:

Human

Cell Background:

Chem-1