Features & Benefits
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Single functional readout – applicable to different GPCRs, NHRs and kinase receptors and families
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Stable cell lines - increased reliability and reproducibility
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Full length protein – ideal for large molecule therapeutics and small molecule inhibitors
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High specificity – tagged protein eliminates background from endogenous proteins
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High quality reproducible data – superior assay windows & performance (Z' > 0.6)
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Simple readout using standard plate readers
Explore Target Biology With Multiple Technology Platforms
Protein:protein Interactions
A homogenous cell-based assay format to detect in vivo interaction of two proteins where the ProLink fragment is attached to one protein and the EA is attached to the second protein. Active β-gal enzyme is formed only when the two proteins interact with each other. The robust assays are specific, sensitive and highthroughput adaptable and use the EFC technology.
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Protein Translocation
PathHunter cells are genetically engineered to over-express the protein under study fused to PL and the EA localized to one of the following organelles: cytoplasm, nucleus, cell membrane, or endosome. Upon compound stimulation of cells, the PL- tagged protein undergoes translocation into the cell compartment in which the EA-is localized. This results in the formation of the active β-gal ezyme which can be detected using a chemiluminescent substrate.
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Protein Degradation
PathHunter protein degradation assays involve a protein of interest tagged with a small ProLink peptide. The assays measure specific ProLink-tagged protein degradation in response to pathway stimulation.
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