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GPCR De-orphanization
In addition to our screening and profiling services, DiscoveRx also offers a unique method in which to deorphanize GPCRs. As a universal G protein-independent assay that generates a receptor specific signal, the PathHunter β-Arrestin platform is ideal for de-orphanization campaigns. Our Orphan Receptor Panel has been successfully used for the identification of novel ligand and receptor pairings. With 77 ready-to-assay oGPCR cell lines, we can deliver full panel screening data in less than 20 days.
Our Service Include
High quality data
Competitive pricing
Complete confidentiality
20 day turnaround time
Universal G-protein independent assay
Access to 77 ready-to-assay oGPCR cell lines
Proven ability to identify receptor: ligand pairs*
To learn more about our service, screening options and pricing, email us at: profiling@discoverx.com
Click on figure to enlarge
Figure 1. PathHunter GPR35 Orphan GPCR Cell Line
(A) EAstern blot showing a band at ~34 kDa in Lane 3, the appropriate size for GPR35 demonstrating that we have
expression and post-translational modification of the receptor.
(B) Dose response curve for Zaprinast,
a "surrogate" ligand for GPR35 showing the expected response to the agonist.
Click on figure to enlarge
Figure 2. Example hit identified in a profile of 275 compounds against a subset of the oGPCR panel demonstrating the utility of PathHunter technology in de-orphanization campaigns.
Available Literature
Click here to view what we have available!
Orphan Receptor Panel
Available Assays and Technologies
We have cell-based assays to address all major drug target classes.
For a complete list of available targets for screening and profiling services, please click on the appropriate links below.
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