HitHunter™ Protease Assays
DiscoveRx's proprietary enzyme fragment complementation (EFC) technology has been adapted to provide a novel method for measurement of proteases. The assay allows for detection of proteolytic activity in-vitro and has an application in screening libraries of compounds for protease inhibitors. The EFC-assay has the advantage of utilizing larger peptide sequences, enabling better recognition of proteases and presents a specific biochemical probe for the protease of interest.
Product Features
•Chemiluminescent assay with amplified signal
•Non-antibody based, homogenous assay
•This technology utilizes longer, natural amino acid sequences (8-30 mer)
•Applicable to endopeptidases, carboxypeptidases and aminopeptidases
For instrument compatibility, please click here.
Applications
•Screen for inhibitors
•Characterize protease, determine Km
•Determine protease activity
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Available Collaterals
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Click here to view similar biochemical assays for kinases, ATPases, GTPases, UTPases