PathHunter™ Cell-Based NHR Assays: Nuclear Translocation Approach*
The assay detects binding of an agonist or an antagonist to the NHR of interest leading to the translocation of NHR to nucleus. The complementation occurs between the two β-galactosidase components: the ProLabel tag which is fused to the NHR of interest; the Enzyme Acceptor (EA) is sequestered into nuclear compartment. These components interact once they are in the same nuclear compartment, forming active β-gal enzyme that converts substrate to detectable signal.
Product Features
•Fewer off-target affects due to shorter assay time (6 hours)
•Assay employs full length NHRs making data more biologically relevant
•High specificity for NHR of interest
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*Covered by issued and pending patent applications. Limited Label License conditions apply. Click here to learn more
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