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PathHunter® Cell-Based NHR Assays: Protein:Protein Interaction*
The assay detects binding of an agonist or an antagonist to the ligand binding site
of NHR of interest and triggering a functional response. Such response is directly
measured using interaction of the NHR to Co-activator protein a prerequisite to
forming a transcriptional complex. Once bound, complementation occurs between
the two β-galactosidase components: the ProLink tag is fused to the C-terminus
of the NHR of interest; the Enzyme Acceptor (EA) is attached to SRCP (Steroid response
co-activator protein). These components interact only when in close proximity,
forming active β-gal enzyme that converts substrate to detectable signal.
Product Features
In vivo whole cell assay
No imaging, antibodies or GFP
No fluorescent interference
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Available Literature
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New! PathHunter® Certified Cell Plating Reagents & Control Ligands
*Covered by issued and pending patent applications. Limited Label License conditions apply. Click here to learn more
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