PathHunter™ Cell-Based NHR Assays: Protein:Protein Interaction*
The assay detects binding of an agonist or an antagonist to the ligand binding site of NHR of interest and triggering a functional response. Such response is directly measured using interaction of the NHR to Co-activator protein a prerequisite to forming a transcriptional complex. Once bound, complementation occurs between the two β-galactosidase components: the ProLink tag is fused to the C-terminus of the NHR of interest; the Enzyme Acceptor (EA) is attached to SRCP (Steroid response co-activator protein). These components interact only when in close proximity, forming active β-gal enzyme that converts substrate to detectable signal.
Product Features
•In vivo whole cell assay
•No imaging, antibodies or GFP
•No fluorescent interference
For instrument compatibility, please click here.
Available Collaterals
click here to view what we have available!
Don't see your target?
Click here to explore our Custom Assay Development possibilities
*Covered by issued and pending patent applications. Limited Label License conditions apply. Click here to learn more
Copyright ©2009, DiscoveRx Corporation. TERMS AND CONDITIONS