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NHR

- Study NHRs Without Time Consuming Reporter Gene Assays

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Nuclear Hormone Receptors (NHRs)

Nuclear Hormone Receptors (NHRs) are a superfamily of ligand-dependent transcription factors that control diverse aspects of growth, development and homeostasis, making them exciting and important targets for drug discovery.

DiscoveRx offers series of assays that measure NHR translocation and NHR-coactivator interaction. These unique assays are chemiluminescent and do not require lengthy 16-24 hour compound incubations characteristic of standard reporter gene assays.

Product Features

Simple, no-wash, non-radioactive assays - Direct 6 hour assays for NHR
activation and signaling
Target-specific cell-based assays or binding assay formats for purified NHRs
Understand effect of compound on multiple pathways with both translocation
and protein:protein interaction approach
Full representation of steroid hormone receptors, reproductive hormones, etc.

NHR Assays Selection Guide

Clonal Cell Lines

PathHunter® Nuclear Translocation Assays

PathHunter® Nuclear Translocation assays are non-imaging whole cell assays to analyze translocation of NHR upon ligand stimulation. Read more»

Targets: GR, AR, LXRα, VDR, MR, PRβ

PathHunter® Protein:protein Interaction

PathHunter® NHR_PRO assays are whole cell functional assays, based on the interaction between NHR and Steroid Receptor Coactivator Peptides (SRCP). The assay is ideal for novel inhibitor screening and pre-toxicity analysis. Read more»

Targets: GR, MR, AR, THRα, THRβ, RARα, RARβ, RXRα, RXRγ, ERRα, LXRα, LXRβ, PPARα, PPARγ, PPARδ, FXR, ERα, PRα, PRβ, RORγ

Clonal Cell Lines

HitHunter® Nuclear
Receptor Assays

Homogeneous immunoassays where analogs of the nuclear hormone compete with ED-nuclear hormone for binding to the nuclear hormone receptor.
Read more»

Targets: PR, ER

Other

PathHunter®
Detection Reagents

Chemiluminescent detection reagents for use with all PathHunter® Cell Lines. Kits available for either Glow or Flash luminescence. Read more»

Don't find your assay?

Talk to our experts, we excel in Custom Assay Development possibilities!
Or you may be interested in NHR screening and profiling services.