HitHunter^® Estrogen and Progesterone Assay

The HitHunter® Estrogen and Progesterone assay are in-vitro based competitive immunoassay for measuring Estrogen or Progesterone analogs that bind to the respective nuclear hormone receptor. It uses EFC detection with a choice of either chemiluminescent or fluorescent intensity readouts.

Product Features

 Directly measure Estrogen or Progesterone competitive analogs
 Adaptable protocols for 96-, 384- and 1536-well applications
 Choice of detection modes:
    – Chemiluminescent or red shifted fluorescent intensity readouts
 Homogeneous assay

How does the assay work?

The HitHunter Nuclear Hormone Receptor Assay Kit utilizes an ED-steroid hormone conjugate in which the steroid hormone is recognized by the steroid hormone receptor. In the absence of free hormone, the ED fragment is incapable of reassociation with EA to form active enzyme and no signal is generated. In the presence of free Ligand, ED-steroid hormone conjugate competes with the free Ligand for receptor binding. The amount of signal is proportional to the amount of nuclear hormone receptor bound.

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Applications

 Determine Estrogen analogs with HitHunter® Estrogen assay
 Determine Progesterone analogs with HitHunter Progesterone assay
 Determine rank order potency of known compounds

Available Literature

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