PathHunter™ Cell-Based Kinase Assays

Receptor Tyrosine Kinases are implicated in disease states such as cancer, metabolic disease, inflammatory disease, cardiovascular and many other disease areas. Biochemical assay uses purified kinase domain, synthetic substrates and lack biological context. A need to understand the kinases in a cellular context prompted DiscoveRx to develop a system to report tyrosine phosphorylation status of proteins in their natural cellular environment. PathHunter™ Cell-Based Kinase assay monitors the interaction between wild type, full length receptor proteins with SH2 phosphotyrosine binding domains. The assay does not require antibodies or washes and can be easily adapted to a one-step HTS-friendly assay.

Product Features

•Ligand induced functional assay
•Whole cell, HTS-friendly, chemiluminescent assay
•Uses full length protein
•No wash, single addition

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Applications

•Find hits from primary and secondary screens
•Screen small molecule inhibitors
•Screen for activating and inhibiting antibodies
•Investigate cell surface interactions

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Available Collaterals

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New! Custom bulk cell options are available with the purchase of any (PathHunter™ Cell-Based Kinase) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.

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