Custom Assay Development

We'll build EFC Assay Tools to Meet Your Needs

Our innovative technology has been successfully applied to many target areas in discovery research.  Custom projects utilize DiscoveRx’s proprietary EFC technology and span both biochemical assays, as well as, functional cell-based assays.

HitHunter^® Biochemical Assays
Kinase Binding Assays
 Conjugate the small fragment of ß-Gal (ED) to your inhibitor
 Characterize and validate binding of an inhibitor to your kinase

Protease Assays
 Develop protease substrates using up to 25 amino acids
 EFC results are independent of peptide length - unlike FRET-based assays

PathHunter^® Cell-Based Assays
ß-Arrestin GPCR Assays
 Direct assay of ligand binding to GPCR
 Universal assay for all GPCR's regardless of G-protein coupling
 Ideal for deorphanization programs

Nuclear Hormone Receptor (NHR) Assays
 Two formats available: Nuclear translocation and Coactivator recruitment
 Multiplex cell lines with both EFC and reporter gene assays in the same cell

Receptor Tyrosine Kinase (RTK) Assays
 Functional cell-based kinase assay
 Based on recruitment of SH2 domain proteins

cAMP Hunter™ GPCR Assays
 Validated cell lines expressing Gi and Gs receptors
 Designed for use with any cAMP detection kit

Don't see your assay?
Contact us at CAD@discoverx.com to discuss your assay needs

 

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