Custom Assay Development

We'll build EFC Assay Tools to Meet Your Needs

Our innovative technology has been successfully applied to many target areas in discovery research.  Custom projects utilize DiscoveRx’s proprietary EFC technology and span both biochemical assays, as well as, functional cell-based assays.

HitHunter™ Biochemical Assays
Kinase Binding Assays
• Conjugate the small fragment of ß-Gal (ED) to your inhibitor
• Characterize and validate binding of an inhibitor to your kinase

Protease Assays
• Develop protease substrates using up to 25 amino acids
• EFC results are independent of peptide length - unlike FRET-based assays

PathHunter™ Cell-Based Assays
ß-Arrestin GPCR Assays
• Direct assay of ligand binding to GPCR
• Universal assay for all GPCR's regardless of G-protein coupling
• Ideal for deorphanization programs

Nuclear Hormone Receptor (NHR) Assays
• Two formats available: Nuclear translocation and Coactivator recruitment
• Multiplex cell lines with both EFC and reporter gene assays in the same cell

Receptor Tyrosine Kinase (RTK) Assays
• Functional cell-based kinase assay
• Based on recruitment of SH2 domain proteins

cAMP Hunter™ GPCR Assays
• Validated cell lines expressing Gi and Gs receptors
• Designed for use with any cAMP detection kit

Don't see your assay?
Contact us at CAD@discoverx.com to discuss your assay needs