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Activity Based Universal Assay for Any Kinase or ATPase
DiscoveRx offers a unique ADP accumulation assay format to study active kinases. ADP Quest™ and ADP Hunter Plus™ family of assay are gain of signal assays,
that are compatible at a range of ATP concentrations, including those at the ATP Km value. The assays employs a generic, non-antibody based, non-radioactive method to
detect the amount of ADP produced during phosphorylation. This unique assay format allows one to identify kinase inhibitors at a constant ATP concentration
Two formats are available, one of which is optimized for research and kinase lead optimization studies (ADP Quest™ and ADP Quest™ HS), while the other is optimized for high-throughput screening of large compound libraries (ADP Hunter™Plus and ADP Hunter™ HS). These approaches employ a proprietary coupled enzyme system, which results in sensitive fluorometric detection of ADP.
Product Features
Kinetic and Endpoint Capability
Can be used with a variety of phosphotransferases such as kinases, ATPases,
UTPases, GTPases. Click here to view validated targets
Compatible with peptide substrates and whole proteins
Homogeneous and non-radioactive assay
For instrument compatibility, please click here.
Available Literature
Click here to view what we have available!
Click here to view receptor tyrosine kinase targets in cell-based format.
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