In this issue:

• PathHunter™ ß-arrestin Assay for GPCRs
• Custom labeling services for Kinase and Protease assays
• Announcements and upcoming conferences
_______________________________________________________________

 

NEW! PathHunter™ ß-arrestin Assays: Homogeneous, Non-imaging assay for virtually any GPCR

PathHunter™ ß-arrestin Assays are revolutionary high-throughput screening assays for monitoring GPCR activation following ligand stimulation, without an imaging instrument, fluorescent protein tag or radioactivity. Instead, the assays detect GPCR activation through movement of ß-arrestin to the expressed GPCR of interest, using EFC technology. The EFC approach offers a range of benefits for screening, including signal amplification, large assay windows, robust performance, and a homogeneous, single-addition assay format. The chemiluminescent signal can be easily read in 96- or 384- microplates with standard luminometers, so library screening is simple, fast, and cost-effective. For additional information, please download the PathHunter™ ß-arrestin flyer. DiscoveRx also offers HitHunter™ cAMP kits for Gs/Gi receptor screening, and Calcium NW, HitHunter™ IP3 assays and Coelenterazine for Gq coupled receptor screening.

Custom Services for Kinase and Protease EFC Assays

DiscoveRx now provides custom labeling services that enable EFC assays for Kinase and Protease screening. For more information on the assays themselves, please download the posters below.

Services include:

1. Labeling of biomolecules with ß-galactosidase Enzyme Donor (ED) tag for use with HitHunter™ Kinase Binding Assays. These assays are ideal for both unactive and active kinases since they measure in a competitve binding assay, the interaction of the inhibitor with the kinase. With DiscoveRx's custom services you can generate binding assays for your own kinase and ligand of choice for unactive and active kinase screening.
   
   

   A Technique using ß-galactosidase (ß-Gal) Enzyme Fragment Complementation (EFC) to Directly Characterize Inhibitor Binding to Active and Unactive Kinases.

   
   
2. Synthesis of Protease substrates for your proprietary protease target based on EFC technology enabling chemiluminescent detection.
   
   

   A Novel Method to Detect Proteases Utilizing Complementation of Enzyme Fragments as a Signal Generation System.

Announcements

For a limited time, get 50% off your order for either SubstrateHunter™ or KinomeHunter™
KinomeHunter™ includes a proprietary, metal based, phosphate binding reagent which provides the flexibility to assay virtually every kinase in the kinome. SubstrateHunter™is a plate containing 40 TAMRA-labeled peptides to quickly identify the ideal serine/threonine peptide substrate for your proprietary kinase.

GE Healthcare Signs Exclusive Distribution Agreement for DiscoveRx HitHunter™ cAMP Assays

DiscoveRx Corporation Unveils a New Strategic Direction

Michael J. Fox Grant awarded to DiscoveRx for Parkinson's Research

Please visit us at:

Society for Biomolecular Screening
Seattle, WA
Sept 17-21, 2006

Scanlab 2006
Copenhagen, Denmark
Sept 26-28 , 2006

American Society for Cell Biology
San Diego, CA
Dec 9-13 , 2006

DiscoveRx – Simple Solutions for Complex Biology