Instruments

Instrument Compatibility

Instrument Compatibility

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Instrument Compatibility Chart

Assay

Instrument

Read-Out

Specifications

ADP Quest™
ADP Quest™ HS
ADP Hunter™ Plus
ADP Hunter™ HS

EnVision- Perkin Elmer
Resorufin/Amplex Red FP 535/40 filter (Cat. #2100-5570)
Emission 595/60 filter
(Cat. #2100-5530)
Victor- Perkin Elmer
Excitation Filter for VICTOR™,
530 nm,
15 mm Diameter, 8 nm Bandwidth
(Cat. #1420-529)
Emission Filter for VICTOR™,
590 nm, 25.4 mm Diameter, 10 nm Bandwidth (Cat. #1420-5860)
ViewLux- Perkin Elmer
Excitation 525 nm Filter, 20 nm Bandwidth (Cat. #11440175) Emission: 598 nm Filter, 25 nm Bandwidth (Cat. #11440174)
Molecular Devices Excitation 535 nm, Emission 590 nm
SpectraMax Gemini XPS/EM - Molecular Devices Excitation: 530 Emission: 590
FlexStation 3 - Molecular Devices: SpectraMax M2/M2e/M3/M4/M5/M5e
Excitation: 530 Emission: 590
PolarStar Optima- BMG
Excitation 544 nm; Emission
590 nm
Tecan Ultra & Spectra Excitation 530 nm Emission 590 nm

Fluorescence

Read Fluorescence Intensity signal:
Resorufin Excitation- 530 nm
Resorufin Emission- 590 nm
Read-time - 1 sec/well

ADP Quest™
ADP Quest™ HS
ADP Hunter™ Plus
ADP Hunter™ HS

Molecular Devices:
SpectraMax® Gemini

Fluorescence

Read Fluorescence Intensity signal:
Resorufin Excitation - 530 nm
Resorufin Emission - 590 nm
Mode: Kinetic

Calcium No
Wash^PLUS

All fluorometers with on-board fluidics
Molecular Devices- FLIPR, FlexStation 3
HAMAMATSU - FDSS

Fluorescence

Excitation 494 nm
Emission 516 nm

Coelenterazine

CYBIO - LUMAX
HAMAMATSU - FDSS
Perkin Elmer: LUMILUX

Flash
Luminescence

CCD imager 1 minute to 5.5 minutes for a 384 well plate

HitHunter^® cAMP
HitHunter^® cGMP
All PathHunter^®
assays
HitHunter^® Kinase Binding Assays

COMPATIBLE WITH ANY LUMINOMETER
BMG: PheraStar, Cytostar, LumiStar
Perkin Elmer: TopCount, Victor 2 or V, Fusion, LumiCount, Envision, Micro-beta (Trilux), Viewlux, Northstar
GE: LEAD seeker, Farcyte
Molecular Devices: FLIPR, SpectraMax M3/M4/M5/M5e, FlexStation 3, SpectraMax L
Tecan: Ultra, Evolution
Turner BioSystems: Modulus Microplate
Caliper LabChip 3000 & EZ Reader
Berthold Technologies- Mithras LB940
Hamamatzu FDS6000, FDSS/RayCatcher
Thermo Scientific: Luminoskan Ascent
Berthold: CentroLIApc
Biotek: Synergy 2

Luminescence

FOR PMT-based luminometer:
GLOW settings
Read the plate for 1 second/well
CCD-based luminometer,
10 - 30 second image

HitHunter^® IP3

BMG: POLARstar Galaxy
Molecular Devices:
Perkin Elmer: Fusion™
Universal & VICTOR2 V
Tecan: ULTRA Evolution
Biotek - Synergy 2
Beckman Coulter MTX 880
plate reader.

Fluorescence Polarization

Excitation Filter Fluorescein- 485 nm
Emission Filter Fluorescein- 530 nm
Dichroic Fluorescein- 505 nm

HitHunter^®
cAMP XS FL

Any standard Fluorescence plate reader

Fluorescence

Excitation 530 ± 25 nm
Emission 610 ± 20 nm

HitHunter
cAMP XS+

Molecular Devices:
SpectraMax® L
Microplate Luminometer

Chemiluminescence

Integration time: 1 second or 0.1 second
Mode: endpoint
PMT sensitivity: Auto Range
Calibration wavelength of 470 nm

HitHunter
cAMP XS+

Index
CHAMELEON V

Chemiluminescence

Integration time: 1 second
Mode: Direct Count

Application Notes

Instrument

Download

ADP Quest™ HS Kinetic Assay with Mithras LB 940

Mithras LB 940

1536 Application for HitHunter^® cAMP XS+ Using
Deerac Equator™ HTS

DEERAC EQUATOR Liwuid dispensor

Evaluation of a new intracellular calcium fluorescent
reagent using the FDSS6000

FDSS6000

HitHunter^® cAMP HS+ assay with Mithras LB 940

Mithras LB 940

New HitHunter^® cAMP XS+ Assay for GPCR screening
Using the PHERAstar

BMG PHERA star