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GPCRs

- Leading Portfolio of Non-force Coupled Cell Lines for Functional GPCR Screening

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Second Messenger Assays

Ligand induced GPCR modulation can be studied by measuring second messenger signaling molecules. G-protein coupled receptor (GPCR) coupled to Gi and Gs activates the cAMP pathway, while GPCR coupled to Gq activates Calcium or IP3. Second messenger assays allow the detection and characterization of all compound classes, full and partial agonist or antagonist, inverse agonist, and allosteric modulators. DiscoveRx offers a full range of cell lines and detection kits for the quantification of these events in assay-ready formats.

Product Features

Homogeneous, non-radioactive assays
Excellent miniaturization capabilities
High sensitivity and high precision
Robust, reliable and rapid detection

Second Messenger Assays Selection Guide

Cell-Based

Second Messenger
Cell Lines

Expedite your screening with stable clonal cell lines expressing Native GPCR.
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cAMP Hunter™ eXpress

Off-the-shelf complete kit format eliminates lengthy cell culture and time consuming assay development. Read More»

Biochemical

HitHunter® cAMP

Large assay windows, reliable, well-proven HitHunter® cAMP technology, fantastic miniaturization capabilities makes these cAMP kits the best in the market.
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HitHunter® IP3

Measure cellular D-Myo-Inositol-1,4,5-trisphosphate (IP3) with HitHunter IP3 assay. Read More»

Calcium No WashPLUS
& Coelenterazine™

DiscoveRx offers solutions for both fluorescent and bioluminescent detection of intracellular calcium. Read More»

Jump-start your screening with industry-leading, well-cited HitHunter® cAMP reagents. Click here for HitHunter® cAMP citations.

Interested in G-protein independent signaling?

Explore PathHunter® β-Arrestin and GPCR Internalization

For a complete list of GPCR targets, click here.