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DiscoveRx: GPCRs - Second Messenger Assays: HitHunter™ cAMP

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GPCR

- HitHunter™ cAMP - Robust, Reliable, Rapid cAMP Detection

HitHunter™ cAMP


HitHunter™ cAMP assays are routinely used in therapeutic campaigns to examine Gi and Gs pharmacology and function. These cAMP assays exhibit robust performance, reproducibility, flexibility and scalability that accelerates and simplifies bench-top assay development to high throughput screening. High tolerance to serum and plasma and broad compatibility to standard plate readers make it the most user-friendly, homogeneous, non-radioactive assay in the market.

View cAMP response on validated, native, non-force coupled Gs and Gi cell lines.


Request your FREE HitHunter™ cAMP sample kit or an on-site demo for FREE!
Click here for more information. Hurry, offer expires August 31, 2010!

Product Features

Largest assay window, ideal for Gi targets
Proven miniaturization to 1536 and 3456
High sensitivity option enables cAMP detection in primary cells
Minimize false positives with cleaner chemiluminescent detection
Positive gain-of-signal assay compatible with any standard plate reader

Applications

Monitoring Gi and Gs targets and identifying agonist, antagonist, allosteric, partial
agonists, inverse agonists
High throughput screening (HTS & uHTS) campaigns
Ligand Profiling
Use with primary cells, orphans, frozen and membrane
Identifying PDE inhibitors

How does it work?


For instrument compatibility, please click here.



Available Collaterals

click here to view what we have available!


HitHunter™ cAMP assays are competitive immunoassays. Free cAMP from cell lysates competes for antibody binding against labeled cAMP (ED-cAMP conjugate). Unbound ED-cAMP is free to complement EA to form active enzyme by EFC, which subsequently hydrolyzes substrate to produce signal. A positive signal generated is directly proportional to the amount of free cAMP bound by the binding protein.



Which kit should I use?


Kits
Catalog #
Key Benefit
EC50 : S/B
Product Insert
Ideal For
HitHunter™
cAMP XS+
90-0075
Largest assay window
30 nM : > 45
Recombinantcells, partial agonist application
HitHunter™
cAMP HS+
90-0090
Highest sensitivity
5 nM : 5
Primary cells, orphans, low levels of cAMP
HitHunter™
cAMP II
90-0034
Shortest protocol
30 nM : 20
Automation
HitHunter™
cAMP MEM
90-0004
Membrane application
40 nM : < 10
Membrane preps
HitHunter™
cAMP
One Step NEW
90-0095
One step
30 nM : 10
Suspension,one step, Gs targets and agonist response
HitHunter™ cAMP XS-FL
90-0081
Fluorescent assay
30 nM : > 30
Recombinant cells, Gs or Gi
92-0005
Validated for HitHunter™ cAMP
cAMP assays

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