HitHunter^® cAMP

HitHunter^® cAMP assays are routinely used in therapeutic campaigns to examine Gi and Gs pharmacology and function. These cAMP assays exhibit robust performance, reproducibility, flexibility and scalability that accelerates and simplifies bench-top assay development to high throughput screening. High tolerance to serum and plasma and broad compatibility to standard plate readers make it the most user-friendly, homogeneous, non-radioactive assay in the market.

View cAMP response on validated, native, non-force coupled Gs and Gi cell lines.

Product Features

 Largest assay window, ideal for Gi targets
 Proven miniaturization to 1536 and 3456
 High sensitivity option enables cAMP detection in primary cells
 inimize false positives with cleaner chemiluminescent detection
 Positive gain-of-signal assay compatible with any standard plate reader

Applications

 Monitoring Gi and Gs targets and identifying agonist, antagonist, allosteric,
    partial agonists, inverse agonists
 High throughput screening (HTS & uHTS) campaigns
 Ligand Profiling
 Use with primary cells, orphans, frozen and membrane
 Identifying PDE inhibitors

How does it work?

For instrument compatibility, please click here.

Available Literature

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HitHunter^® cAMP assays are competitive immunoassays. Free cAMP from cell lysates competes for antibody binding against labeled cAMP (ED-cAMP conjugate). Unbound ED-cAMP is free to complement EA to form active enzyme by EFC, which subsequently hydrolyzes substrate to produce signal. A positive signal generated is directly proportional to the amount of free cAMP bound by the binding protein.

Which kit should I use?

 

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