cAMP Hunter™ eXpress GPCR Kits
Fast and Simple Second Messenger Assays without Force Coupling
cAMP Hunter™ eXpress GPCR Assays provide a fast and simple second messenger assay for monitoring activation of even the most difficult GPCR targets in live cells. Unlike other force-coupled technologies, cAMP Hunter eXpress cells utilize the natural coupling status of the GPCR to monitor activation of Gi- and Gs-coupled receptors.
Product Features
•Over 51 human Gi- and Gs-coupled GPCRs available
•Off-the-shelf complete kit format
•Pre-validated cells and reagents
•Optimized step-by-step protocol
•Assay-Ready frozen cells
•Non-radioactive
•Read on any standard benchtop luminometer
How the cAMP Hunter™ eXpress GPCR Assays Work
GPCR activation following ligand binding initiates a series of second messenger cascades that result in a cellular response. This signaling involves a protein called adenylate cyclase (AC). Gαi and Gαs-coupled receptors modulate cyclic Adenosine Monophosphate (cyclic AMP or cAMP) by either inhibiting or stimulating AC, respectively.
The cAMP Hunter eXpress GPCR assays monitor the functional status of the GPCR by measuring cellular cAMP levels using a homogeneous, gain-of-signal competitive immunoassay based on Enzyme Fragment Complementation (EFC). Thus, the EFC signal generated is directly proportional to the amount of cAMP produced by receptor activation.
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Available Collaterals
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Kit Contents
•Assay-ready, frozen cAMP Hunter™ eXpress cells
•(2 vials each, 3.75 x 106 cells/vial)
•Optimized Cell Culture Medium
•Cell Assay Buffer
•cAMP Hunter Detection Reagents
•Dried Forskolin
•96-well Tissue Culture Treated Plates