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cAMP Hunter™ eXpress GPCR Kits
Fast and Simple Second Messenger Assays without Force Coupling
cAMP Hunter™ eXpress GPCR Assays provide a fast and simple second messenger assay for monitoring activation of even the most difficult GPCR targets in live cells. Unlike other force-coupled technologies, cAMP Hunter eXpress cells utilize the natural coupling status of the GPCR to monitor activation of Gi- and Gs-coupled receptors.
Product Features
Over
51 human
Gi- and Gs-coupled GPCRs available
Off-the-shelf complete kit format
Pre-validated cells and reagents
Optimized step-by-step protocol
Assay-Ready frozen cells
Non-radioactive
Read on any standard benchtop luminometer
How the cAMP Hunter™ eXpress GPCR Assays Work
GPCR activation following ligand binding initiates a series of second messenger cascades that result in a cellular response. This signaling involves a protein called adenylate cyclase (AC). Gαi and Gαs-coupled receptors modulate cyclic Adenosine Monophosphate (cyclic AMP or cAMP) by either inhibiting or stimulating AC, respectively.
The cAMP Hunter eXpress GPCR assays monitor the functional status of the GPCR by measuring cellular cAMP levels using a homogeneous, gain-of-signal competitive immunoassay based on Enzyme Fragment Complementation (EFC). Thus, the EFC signal generated is directly proportional to the amount of cAMP produced by receptor activation.
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Available Literature
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Download the user manual 
Kit Contents
Assay-ready, frozen cAMP Hunter™ eXpress cells
(2 vials each, 3.75 x 106 cells/vial)
Optimized Cell Culture Medium
Cell Assay Buffer
cAMP Hunter Detection Reagents
Dried Forskolin
96-well Tissue Culture Treated Plates
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