Calcium Detection

Measurement of intracellular calcium provides valuable information on the activation status of GPCRs and ion channels. HTS-friendly calcium assays include fluorescent based assays and photoprotein based assays. The DiscoveRx Calcium No Wash^PLUS (Ca NW^PLUS) assay kit provides a fast, reliable, low cost alternative to traditional wash and quench-based assays for detection of changes in intracellular calcium in response to GPCR activation. View native non-force coupled Gq cell lines validated with this detection kit.

Calcium No Wash^PLUS

Product Features

•No wash, one step protocol
•Improved signal and better performance than FLUO-3
•Compatible with almost all fluorometers with on-board fluidics

Assay Principle

Applications

•Assess Gq-coupled GPCRs
•Ideal for calcium screening for agonist or antagonists
•Well-suited for both native non-force coupled GPCRs and force coupled targets
•View data on
– Calcium response in native Gq coupled PathHunter™ β-Arrestin cell lines
– Sequential determination of Calcium and arrestin read-out in a single well
– Calcium and cAMP multiplex

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Aequorin-Based Calcium Assays

On the other hand, the aequorin-based luminescence assays require coelenterazine. Coelenterazine h (90-0084), provided by DiscoveRx is a derivative of native coelenterazine that is more sensitive to calcium, making it an ideal choice for luminescent calcium HTS.

Product Features

•Luminescent signal gives you low false positives
•Fast, simple protocol
•Large assay windows
•20-fold more sensitive than native coelenterazine

For instrument compatibility, please click here.

Available Collaterals

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