PathHunter™ β-Arrestin Assays
A wide variety of assays are available for analyzing GPCR signaling events, many
of them based on second messenger analysis. PathHunter™ β-Arrestin assays are
unique in that they detect primary rather than secondary activation events. The
assays provide a direct measure of GPCR activation by detecting the binding of
β-Arrestin to the GPCR of interest. Because Arrestin recruitment occurs independent
of G-protein coupling, the PathHunter™ β-Arrestin assays offer a powerful and
universal screening platform that can be used with virtually any Gi-, Gs-, or Gq-coupled
receptors.
How the PathHunter β-Arrestin Works
In this system, β-Arrestin is fused to an N-terminal deletion mutant of β-gal (termed the enzyme acceptor of EA) the GPCR of interest is fused to a smaller (42 amino acids), weakly complementing fragment termed ProLink™. In cells that stably express these fusion proteins, ligand stimulation results in the interaction of β-Arrestin and the ProLink-tagged GPCR, forcing the complementation of the two β-gal fragments and resulting in the formation of a functional enzyme that converts substrate to detectable signal.
Product Features
•Over 220 validated Gi, Gs, and Gq-coupled GPCR assays
•Direct measure of GPCR activation state via β-Arrestin recruitment, independent
of G-protein signaling
•Human, Mouse and Rat GPCR assays available
•Ideal for de-orphanization
•Simple, non-radioactive single addition protocol optimized for a HTS-friendly format
•HTS-friendly format, easily miniaturized
For instrument compatibility, please click here.
Available Collaterals
click here to view what we have available!
PathHunter™ Technology Available In Several Formats
Cell Lines
Choose from over 220 fully validated cell lines. Click here
Orphan Cell Lines
β-Arrestin GPCR Assays
β-Arrestin GPCR Assays
Detection Reagents
β-Arrestin Cell Lines. Kits available for either Glow or Flash luminescence. Read More»
Toolbox Products
*Covered by Issued and Pending patents. Limited Label License Conditions apply. Click here to learn more.
Click here to view a complete list of GPCR targets.
Don't see your target?
Click here to request our Custom Assay Development Service
Don't want to do cell culture?
New! Bulk cell options are available with teh purchase of any PathHunter clone.
Package sizes range from 100 million to 3 billion cells. Bulk cells are functionally
tested for optimal assay performance and are confirmed to be Mycoplasma-free.
Combine frozen, assay-ready bulk cells with PathHunter-Approved Cell Plating
Reagent and Control Ligand to expedite your screening campaigns and ensure
optimal and reproducible results every time.
Click here for a quote.
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