In this issue:

• Recent Developments - PathHunter™ Assays
• Special Offers
• Presentations and Publications
• Announcements and conferences
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Recent developments with PathHunter™ Assays:

PATHHUNTER™ ASSAYS: RECIPIENT OF THE 2006 FROST & SULLIVAN TECHNOLOGY INNOVATION AWARD!

PathHunter™ ß-arrestin Assays are revolutionary high-throughput screening assays for monitoring G-protein coupled receptor (GPCR) activation following ligand stimulation without an imaging instrument, fluorescent protein tag, or radioactivity. Instead, the assays detect GPCR activation through ß-arrestin binding to the expressed GPCR of interest, measuring the interaction of the two proteins using enzyme fragment complementation.  In addition to a series of ß-arrestin cell lines and ProLink vectors, DiscoveRx is now offering custom cell pool generation to help you test and validate PathHunter ß-arrestin assays for your specific GPCR of interest.

The technology has  been successfully validated with many GPCRs, including the following:

• B2AR (beta 2 adrenergic receptor), SSTR2 (somatostatin receptor), CB1 (cannabinoid receptor), EDG1 (sphingosine-1-phosphate receptor), M5 (muscarinic receptor), HRH2 & HRH4 (histamine receptors), D1 (dopamine receptor), MC4R (melanocortin receptor), and AVPR2 (vasopressin receptor 2)

For more information, please download the PathHunter™ ß-arrestin flyer or request a seminar or on-site demonstration.

In addition, many new ProLabel expression vectors have been released for the PathHunter technology platform.  This means faster assay development time for you! Please contact us for details.

Special Offers:

Take the Calcium No Wash challenge!  Compare us head to head with your other calcium kits by requesting a free demo.

ADP Hunter™ PLUS is a truly generic, homogenous kinase assay that works with both peptide and whole protein substrates, and unlike ATP depletion assays has a positive readout. Try us for your next kinase screen and receive a discount on your first order!

Upcoming Presentations at SBS:

Tutorial session:
An improved, generic screening approach for GPCR targets utilizing ß-arrestin and enzyme fragment complementation (EFC)
Keith Olson, VP of Product Development
Mon, Sept 18th at 9 am
Room 620

Plenary session:
Biochemical & Cellular Assay Development Using ß-Galactosidase Complementation
Keith Olson, VP of Product Development
Mon, Sept 18th at 2:40 pm
Room 6B

DiscoveRx will also be presenting the following posters at SBS 2006:

1. Development of a non-imaging, homogeneous assay format for analysis of arrestin recruitment as a detection method for generic GPCR screening

2. A technique using b-galactosidase (b-Gal) enzyme fragment complementation (EFC) to directly characterize inhibitor binding to active and unactive kinases

3. Application of a homogeneous, non-imaging cell-based assay for rapid and direct detection of NHR translocation

4. Utilization of a mitotic index biosensor in a homogeneous assay format for identification of siRNAs that inhibit mitosis

5. An optimized assay format with minimized compound interference for kinase primary screening using ADP accumulation

6. A Novel Calcium No Wash Assay for Analysis of GPCR Signaling
   

Additional posters highlighting DiscoveRx technology at SBS 2006:

1. An analysis of the Mitotic Index Assay with respect to cell toxicity in a high throughput screening format; Myron Srikumaran, Abbot Laboratories

2. A fully automated, homogeneous [35S]GTPgammaS SPA binding assay in 1536-well plate format; Sarah Shi, Merck & Co.

3. Comparison of no wash dyes for FLIPR calcium mobilization assays; Donna Terenzio, Boehringer Ingelheim Pharmaceuticals, Inc.

4. Screening for HsEg5 Inhibitors using ArQule's Proprietary Libraries; Bin Zhang, ArQule Inc.

4. Identification of Small Molecule Allosteric Modulators Downregulating Gs- and Gi-coupled GPCRs by Enzyme Fragment Complementation Assay; Han Xu, Amgen

New Publications of DiscoveRx Technology:

A homogeneous cell-based assay to measure nuclear translocation using beta-galactosidase enzyme fragment complementation, Assay Drug Dev Technol. 2006 Jun;4(3):263-72

Drug Discovery: Versatile Tag for Cell-Based Screening, Genetic Engineering News.  Aug 1 2006 (Vol. 26, No. 14)  

Announcements:

DiscoveRx PathHunter™ Technology Successfully Implemented in NIH Roadmap Initiative

 

Society for Biomolecular Screening Seattle, WA Sept 17-21, 2006 Come visit us at booth #2601 and register to win a T-Mobile SideKick cell phone!

American Society for Cell Biology
San Diego, CA
Dec 9-13, 2006

 

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