ProLink™ Detection Kit

The ProLink™ Detection Kit is used to confirm expression of ProLink-tagged proteins. ProLink (PK or ED) is a components of DiscoveRx's β-gal complementation technology and is used to engineer all PathHunter™ protein-protein interaction cell lines, including β-Arrestin GPCR cell lines, Receptor Tyrosine Kinase cell lines, Nuclear Hormone Activation cell lines and others. The asssay has been validated for use in both 96-well and 384-well microplate formats. This kit is a companion product for DiscoveRx's PathHunter™ Product Line.

Applications

• All PathHunter™ Assays
• All PathHunter™ eXpress Assays
• All PathHunter™ Orphan eXpress Assays
• All PathHunter™ Receptor Tyrosine Kinase Assays
• All PathHunter™ Nuclear Hormone Receptor Assays
• Any other PathHunter™ protein:protein Interaction Assay

Representative Data:
Example: Confirm Expression of Orphan GPCRs

DiscoveRx's Orphan GPCR Cell Lines, GPR88 (part number: 93-0357C2A) and ADMR (part number: 93-0403C2) were anayzed using this kit to confirm expression of the respective GPCR-PK fusion proteins.

These data demonstrate that GPCR88-PK and ADMR-PK are expressed at abundant levels because of the significant Raw Luminescence Unit (RLU) difference between the dilution buffer and the EA reagent. In general, the ratio of EA Reagent/Dilution Buffer RLU values for any particular cell line expressing a PK-tagged protein should
be ≥ 2.

Current Ligand List

 

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