ProLink™ Vectors

 

Build Protein Interaction Assays with our ProLink Cloning and Expression Vectors

ProLink Cloning Vectors: ProLink Cloning Vectors are designed such that the gene of interest is cloned in frame with the weakly complementing fragment of β-gal termed ProLink (PK). ProLink is a low affinity tag (~30-fold lower affinity for the Enzyme Acceptor (EA) than the ProLabel) used for construction of protein-protein interaction assays, such as GPCR: β-Arrestin. The ProLink2 (PK2) tag has higher affinity for EA than the original ProLink (PK) tag (~3-fold greater) and is useful for weak protein:protein interactions. For the construction of GPCR cell lines, we have specialized vectors which can improve the S:B ratio. G-protein Receptor Kinase (GRK) phosphorylates GPCRs; facilitating recruitment of arrestins. The Arrestin Recruitment Modifying Sequence (ARMS) is a GRK consensus phosphorylation site deduced from analysis of GPCRs which generate a high signal:background in the PathHunter assay. The addition of ARMS can improve assays which have a low S:B ratio. All ProLink Cloning vectors utilize a minimal CMV promoter for effective expression in mammalian cells.

Choose a ProLabel or ProLink Expression Vector: ProLabel and ProLink Expression Vectors are available with the 4 kDa tag on a wide variety of proteins.

Product Features

•Small, 4 kDa ProLink tag is less likely to perturb normal protein function
•Develop novel assays to identify the effects of siRNA, therapeutic antibodies,
 small molecules, etc.

For instrument compatibility, please click here.

(click on figure to enlarge)
Figure 1. Map of the pCMV-PK and pCMV-PK2 Cloning Vectors.

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Figure 2. Map of the pCMV-ARMS1-PK2 and pCMV-ARMS2-PK2 Cloning Vectors.

Cloning Vectors

Expression Vectors

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