PathHunter™ EA and ProLabel™ Detection Kits

The PathHunter™ EA Detection Kit is used with PathHunter™ Cell Lines that express the Enzyme Acceptor(EA) fragment of β-galactosidase, such as EA-Nuc, EA-Cyto
or EA-Arrestin Parental Cell Lines. This detection reagent formulation includes ED reagent, a small ProLabel/ProLink-type peptide that complements with EA during assay incubation.

The PathHunter ProLabel Detection Kit is for use with cell lines expressing ProLabel (PL) or ProLink (PK) expression or cloning vectors. The kit measures the total amount of ProLabel or ProLink-tagged protein expressed in cells.

NOTE:  PathHunter Cell Lines expressing both a Prolabel or ProLink-tagged protein and EA require the PathHunter™ Detection Kit (93-0001).

Product Features

•Simple, homogeneous assay for detection of either EA or PL/PK expression in cells
•Confirm expression of proteins without the necessity of using an antibody against
for your target protein

For instrument compatibility, please click here.

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Figure 1. EFC activity of PathHunter Cell Lines
The PathHunter HEK 293 EA-Arrestin Parental Cell Line and PathHunter CHO-K1 EA-Nuc Parental Cell Line were seeded at 5,000 cells per well into a 384-well plate. EFC activity was measured using the PathHunter EA Detection kit according to the assay procedure. A 10-fold increase in signal over background was observed and indicated strong expression of EA in both cell lines. The EA positive control provided produced a comparable signal when used at a 100-fold dilution.

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Figure 2. EFC activity of CHO-K1 Cell Line expressing IκBα-ProLabel fusion protein
A CHO-K1 cell line expressing IκBα-PL fusion protein was seeded into a 384-well plate at 5,000 cells per well. EFC activity was measured using the PathHunter ProLabel Detection Kit according to the assay procedure. A 30-fold increase in signal over background was observed and indicated good expression of the fusion protein in the cells. The PL provided control peptide generated a comparable EFC signal at a 50-fold dilution.