HitHunter™ Cortisol^PLUS Assay

Cortisol is a steroid hormone that is produced and secreted by adrenal cortex. It plays an important role in the regulation of blood pressure and cardiovascular function as well as regulation of the body's metabolism of proteins, carbohydrates, and fats. HitHunter™ Cortisol^PLUS Assay is a reliable and simple method for the detection of cortisol. It is designed to be extremely flexible for a variety of formats suitable for discovery research for both therapeutic and HTS programs.

Product Features

•Compatible with cells or purified enzyme preparations
•HTS-friendly, chemiluminescent assay
•Specific Antibody with very low cross-reactivity
•Robust assays with large assay windows

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Applications

•Directly measure cortisol or cortisol expressed from microsomes
•Ideal for inhibitor screening of 11β-hydroxysteriod dehydrogenase type 1 and 2
isoenzyme activity

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Available Collaterals

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Measuring cortisol using HitHunter EFC Detection

HitHunter Cortisol Plus Assay is a rapid, homogeneous method for measuring cortisol using Enzyme Fragment Complementation (EFC) as the detection method. Free cortisol competes for antibody binding against labeled ED-cortisol conjugate, a small peptide fragment of β-galactosidase (β-gal). Unbound ED-cortisol is free to complement with EA, an inactive β-gal EFC enzyme, to form active enzyme, which subsequently hydrolyzes luminescent substrate for EFC detection by a microplate reader or CCD camera. The amount of signal generated is proportional to the amount of cortisol that is unbound by antibody.