| Biological Information | |
|---|---|
| Background Information: | The PathHunter® SIRPa Signaling Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. Engagement of the receptor by its ligand (e.g. CD-47 or agonist antibodies) results in activation of the SIRPα-ED fusion protein, which leads to the recruitment of SHP-1-EA, forcing complementation of the EFC components to create an active β-gal enzyme. This active enzyme hydrolyzes substrate to create a chemiluminescence signal as a measure of receptor activity. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. |
| Target Class: | Checkpoint Receptor |
| Family: | IgSF (immune inhibitory Receptor) |
| Accession Number: | NM_001040022.1 |
| Target Name: | SIRPa |
| Target Aliases: | SIRPA, Signal regulatory protein alpha, BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1 |
| Target Species: | Human |
| Cell Background: | Jurkat |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | Signaling |
| Detection Method: | Chemiluminescence |
| Clinical Relevance | |
| Therapeutic Area: | Oncology/Immuno-Oncology |
| Additional Information | |
| Brand: | PathHunter® |
PathHunter® Jurkat SIRPa Signaling Bioassay Kit
The PathHunter® SIRPa Signaling Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. Engagement of the receptor by its ligand (e.g. CD-47 or agonist antibodies) leads to the recruitment of SHP-1-EA
User Manuals & Protocols
70-411 PathHunter Jurkat SIRPa Signaling Bioassay Kit User Manual REV2
View DocumentDatasheets
93-1135Y19x Datasheet
View Document