Find a Product

KILR® Retroparticles for Adherent Cells (G418)

KILR® Retroparticles for Adherent Cells (G418) contains MMLV particles, that can transduce a wide variety of adherent cells, resulting in high level expression with a housekeeping gene tagged with β-gal reporter fragment, ProLabel® (ePL). This results in a high level of expression of the fusion protein inside the target cells. Target cell death in a cytotoxicity assay results in the release of the ePL-tagged protein into the medium. Addition of KILR detection reagent, containing the complementing β-gal reporter fragment, EA, results in complementation of the two enzyme fragments (EA and ePL). The resulting functional enzyme hydrolyzes a substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
97-00034 Vials x 0.5 mL$4279.00

For account specific pricing, please contact Customer Service at info@discoverx.com.
Cryovial Label
KILR Retroparticles (G418) - Adherent
Vial Contents
0.5 ml, > 5 x 106 cfu/ml
Storage Conditions
Short term (<24 h): Store at -80°C; Long term (>24 h): Store in vapor phase of liquid nitrogen.
Recommended Use
Transduction of adherent cells to generate KILR cell lines, for use in immune-mediated cytotoxicity assays
  • [User Manual] KILR® Retroparticles For Cytotoxicity Assays

    The KILR Retroparticles provide a potent vehicle for the stable delivery of the KILR reporter construct into almost any dividing cell line, enabling the creation of KILR cytotoxicity assays in the cell line expressing the target of your choice. The KILR Cytotoxicity cell line you generate enables the development of a simple, non-radioactive and dye-free method ...

    [User Manual] KILR® Retroparticles For Cytotoxicity Assays

Assay Principle




The KILR retroviral particle is a Moloney Murine Leukemia Virus (MMLV) engineered to drive expression of the KILR reporter construct, a housekeeping gene tagged with Enhanced ProLabel® (ePL), a β-galactosidase (β-gal) reporter fragment. The KILR Retroparticles for Suspension cells contain a hybrid LTR promoter for expression of the fusion protein in difficult-to-transduce suspension cell lines, whereas the KILR Retroparticles for Adherent cells use a CMV promoter for expression of the KILR construct in most adherent cell lines. When stably transduced with the KILR Retroparticles, the target cells express the KILR reporter construct inside the cells. Death of the cells leads to rupture of the cell membrane and the KILR reporter protein is released into the medium. Addition of KILR detection reagent, containing the complementing β-gal reporter fragment, Enzyme Acceptor (EA), results in complementation of the two enzyme fragments (EA and ePL; See Figure 1). The resulting active enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Applications of the KILR Cytotoxicity Assays


1. Antibody dependent cell-mediated cytotoxicity (ADCC)
2. Complement dependent cytotoxicity (CDC)
3. Antibody dependent cell phagocytosis (ADCP)
4. Cytotoxic t-cell lymphocyte mediated death (CTL)
5. Bi-specific antibody mediated T-cell redirection
6. Chimeric antigen receptor t-cell (CAR-T)
7. Adoptive T-cell therapies

Required Products

Cat. No. Description Size Price Quantity
97-0001M KILR® Detection Kit 1,000 dp (96-well) $1263.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $89.00