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pCMV-ProLink™ 1 Vector

DiscoverX cell-based assays feature the powerful in vivo application of the established Enzyme Fragment Complementation technology. In this approach, two complementing fragments (ProLink, a small enzyme donor peptide fragment, and EA, a larger enzyme acceptor fragment) of the β-galactosidase (β-gal) enzyme are expressed as protein fusions in stably transfected, clonally derived cells. The pCMV-ProLink™ cloning vectors are intended for cloning a GPCR or other protein of interest with ProLink as a C-terminal tag, and subsequently transfecting into a parental cell line containing an EA-tagged protein. ProLink 1 peptide tag has low affinity for EA and is useful for strong protein:protein interactions, while ProLink 2 peptide tag has a higher affinity (~3-fold greater) for EA and is useful for weak protein:protein interactions. ARMS (Arrestin Recruitment Modifying Sequence) ProLink tags contain GRK (G-protein Receptor Kinase) consensus phosphorylation sites and can improve assays that have a low signal-to-background ratio.

Catalog No. Size Price Quantity
93-0167 1 Vector $659.00

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Description
pCMV-ProLink™ 1 Vector
Contents
1 vial, 10µg in 20µL
Target
PK1
Compound
Compound
Compound Type
CompoundType
Storage
Ships frozen on dry ice. Store at –20°C until use.
Molecular WT (g/mole)
MolecularWTgmole
Source
Source
FigureLegend
  • [User Manual] ProLabel® and ProLink™ Mammalian Cloning Vectors

    pCMV Mammalian cloning vectors are intended for cloning your protein of interest (e.g. membrane protein or intracellular protein) with ePL, PK1 or PK2, as a N- or C-terminal tag, and subsequently transfecting into a parental cell line expressing an EA-tagged reporter protein (Note: in some assay formats the cells only express an ED protein and the EA protein is...

    [User Manual] ProLabel® and ProLink™
Mammalian Cloning Vectors