Ready-to-use AssayComplete Hygromycin B is a sterile-filtered, selection antibiotic used for the selection and maintenance of eukaryotic cells transfected with the E. coli Hygromycin resistance gene. The effective killing concentration of this antibiotic will vary with the cell type, media, growth conditions, density, and the cell’s metabolic rate and position in the cell cycle. For effective concentrations of Hygromycin B to be used in cell culture kits, please refer to cell line specific datasheet.