PathHunter® Flash Detection Kit

The PathHunter Flash Detection Kit is to be used with PathHunter Cell Lines or cell lines made with ProLabel®/ProLink™ expression or cloning vectors. The kit is easy-to-use and has been successfully run in both 96-well and 384-well microplate format. The resulting signal is chemiluminescent and is read with any standard plate reader. PathHunter products utilize the Enzyme Fragment Complementation (EFC) technology for studying proteinprotein interactions, protein translocation, receptor trafficking, and receptor internalization, all of which involve the complementation of two β-galactosidase (β-gal) fragments. The larger β-gal fragment is called Enzyme Acceptor (EA) and the smaller fragment is termed Enzyme Donor (ED; also known as ProLink or ProLabel). The two fragments are inactive when apart. However, when they complement, they form a functional enzyme that hydrolyzes the substrate to generate a chemiluminescent signal. The detection reagents do not contain either the EA or ED fragments of β-gal, and therefore must be used in conjunction with cells that express both components in order to obtain a detectable signal.

Catalog No. Size Price Quantity
93-0247L 1,000 mL (100 plates), 40,000 dp, (384-well) Enquire
93-0247M 25 mL (2 plates), 200/800 dp (96-well/384-well) $839.00
93-0247 125 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $3,479.00

Please contact Customer Service at customerservicedrx@eurofins.com for European (EUR) or United Kingdom (GBP) pricing or log in to see your account-specific pricing.
Description
PathHunter® Flash Detection Kit
  • [User Manual] PathHunter® Flash Detection Kit

    The PathHunter Flash Detection Kit is to be used with PathHunter Cell Lines or cell lines made with ProLabel®/ProLink™ expression or cloning vectors. The kit is easy-to-use and has been successfully run in both 96-well and 384-well microplate format. The resulting signal is chemiluminescent and is read with any standard plate reader.
    [User Manual] PathHunter® Flash Detection Kit