ChemiScreen™ GAL2 Galanin Receptor Membrane Preparation

Galanin is a 29-30 amino acid peptide originally purified from intestine, but later found to be abundant in the CNS. It is widely distributed in tissues such as the brain, spinal cord and gut, and can regulate numerous processes including feeding, nociception, nerve regeneration, memory, neuroendocrine release, and gut secretion and contractility. Galanin elicits its physiological effects through the stimulation of at least three G protein-coupled receptors (Branchek et al. 2000). GAL2 receptor couples predominantly to the activation of phospholipase C. It plays an important role in modulating neurite outgrowth and has been demonstrated to be the principal receptor subtype that mediates the protective effects of galanin in the hippocampus (Elliott-Hunt et al. 2007). GAL2 receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GAL2 receptor interactions. The membrane preparations exhibit a Kd of 1.4 nM for [125I]-Galanin. With 10 mg/well GAL2 receptor Membrane Prep and 0.5 nM [125I]-Galanin, a greater than 20-fold signal-to-background ratio was obtained.

Catalog No. Size Price Quantity
HTS186M 1 Vial $779.00

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Application
Radioligand Binding Assay
Family
Galanin
Target
GAL2
Accession Number
NM_003857
Species
Human
Host Cell Type
Chem-1
Content
1 Vial (1 mL) for two 96-well plates or 200 units
Storage
Vial to be stored at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.