ChemiScreen™ PK2 Prokineticin Receptor Membrane Preparation

Prokineticins, also known as endocrine gland vascular endothelial growth factors (EG-VEGF), are two ~10 kD secreted proteins originally described to mediate angiogenesis and gastrointestinal smooth muscle contraction (Li et al., 2001; LeCouter et al., 2003). Subsequently, prokineticins have been found to mediate central nervous system functions including circadian rhythms and olfactory bulb development (Cheng et al., 2002; Ng et al., 2005). Two Gq-coupled receptors, PK1 and PK2 (also known as GPR73a and GPR73b), mediate cellular responses to prokineticins (Lin et al., 2002). PK2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at PK2. The membrane preparations exhibit a Kd of 0.36 nM for [125I]-MIT-1. With 0.3 nM [125I]-MIT-1, 10mg/well PK2 Membrane Prep typically yields a 2-fold signal-to-background ratio.

Catalog No. Size Price Quantity
HTS182M 1 Vial $779.00

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Radioligand Binding Assay
Host Cell Type
1 Vial (1 mL) for two 96-well plates or 200 units
Vial to be stored at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.