ChemiScreen™ NPBW1 Neuropeptide B/W Receptor Membrane Preparation

Neuropeptide B (NPB) and neuropeptide W (NPW) are members of a recently identified neuropeptide family that are ligands for two highly similar receptors NPBW1 and NPBW2, both of which are coupled to Gi/o protein to inhibit intracellular cAMP production. Highest expression of NPBW1 mRNA and protein was identified in the amygdala and hypothalamic nuclei. Physiological studies demonstrate that intracerebroventricular infusion of NPBW1 ligands modulates feeding behavior, regulates the release of corticosterone, prolactin and growth hormone while also modulating pain pathway (Singh and Davenport, 2006). NPBW1 knock out male mice have shown mild adult-onset obesity and decreased locomotor activity. They become progressively hyperglycaemic and hyperinsulinaemic (Ishii et al., 2003). NPBW1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NPBW1 receptor interactions with its ligand. The membrane preparations exhibit a Kd of 0.53 nM for [125I]-Neuropeptide W23. With 5 ug/well of NPBW1 Membrane Prep and 0.35 nM [125I]-Neuropeptide W23, a greater than 10-fold signal-to-background ratio was obtained.

Catalog No. Size Price Quantity
HTS180M 1 Vial $779.00

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Application
Radioligand Binding Assay
Family
Neuropeptide B & W
Target
GPR7
Accession Number
NM_005285
Species
Human
Host Cell Type
Chem-1
Content
1 Vial (1 mL) for two 96-well plates or 200 units
Storage
Vial to be stored at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.