ChemiScreen™ PRP/GPR10 Prolactin-Releasing Peptide Receptor Membrane Preparation

PRP, also known as GPR10 or hGR3, is a Gq-coupled receptor for prolactin-releasing peptide that is expressed in the pituitary (Hinuma et al., 1998). Genetic studies in rodents indicate that lack of GPR10 leads to hyperphagia, obesity and dyslipidemia (Gu et al., 2004; Watanabe et al., 2005). In humans, genetic variations in GPR10 are associated with lowered blood pressure (Bhattacharyya et al., 2003). GPR10/PRP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GPR10/PRP interactions with prolactin-releasing peptide. The membrane preparations exhibit a Kd of 0.59-0.66 nM for [125I]-PRP-20. With 5 mg/well PRP Membrane Prep and 0.25 nM [125I]-PRP-20, a greater than 30-fold signal-to-background ratio was obtained.

Catalog No. Size Price Quantity
HTS057M 1 Vial $779.00

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Application
Radioligand Binding Assay, GTPγS Binding
Family
Prolactin Releasing Peptide
Target
PRRP
Accession Number
NM_004248
Species
Human
Host Cell Type
Chem-1
Content
1 Vial (1 mL) for two 96-well plates or 200 units
Storage
Vial to be stored at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.