ChemiScreen™ CRTH2/DP2 Prostanoid Receptor Membrane Preparation

Chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) is a GPCR related to FPR-like chemoattractant receptors that is highly expressed on Th2 cells, eosinophils, basophils, and type 2 cytotoxic T cells (Nagata et al., 1999). The most potent ligand for CRTH2 is prostaglandin D2 (PGD2), a major mast cell-derived allergic mediator (Hirai et al., 2001). Another GPCR, DP, is activated by PGD2, and the relative contributions of DP and CRTH2 to PGD2-mediated allergic disease are being elucidated. A small molecule antagonist of CRTH2, ramatroban, attenuates PGD2-mediated bronchial hyperresponsiveness and antigen-induced inflammation (Sugimoto et al., 2003; Shichijo et al., 2003). The cloned human CRTH2 expressing cell line is made in the Chem-5 host, which supports high levels of CRTH2 expression on the cell surface and contains high levels of the promiscuous G proteins to enhance coupling of the receptor to the calcium-signaling pathway. The cell line is an ideal tool for screening antagonists of interaction between CRTH2 and its ligand.

Catalog No. Size Price Quantity
HTS031M 1 Vial $779.00

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Application
Radioligand Binding Assay, GTPγS Binding
Family
Prostanoid
Target
DP2/CRTH2
Accession Number
AB008535
Species
Human
Host Cell Type
Chem-5
Content
1 Vial (1 mL) for two 96-well plates or 200 units
Storage
Vial to be stored at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.