Ready-to-Assay™ GPCR frozen cells are designed for simple, rapid assays with no requirement for intensive cell culturing. Eurofins DiscoverX Products LLC. has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. Simply thaw the cells and resuspend them in media, dispense cell suspension into assay plates and, following overnight recovery follow the protocol to run the assay.
Cloned HEK Parental ChemiBrite with Gαqo cells were made by stable transfection of HEK293 cells with optimized quantities of plasmid encoding a novel variant of clytin and human Gαqo. These stability-tested cells are ideal for recombinant expression of target protein for use in calcium flux assays, for analysis of agonist, antagonist and modulator activity at the target protein, as well as cAMP assays.